Abstract: Five types of explants including young embryo ,young inflorescence ,shoot tips，kernel and mature embryo were cultured . But regenerating ability varies greatly with the different explant types, among which calli from young inflorescence and young embryo has the best regenerating ability. Somaclonal variations were investigated in 166 R2 lines derived from nine genotypes .The results revealed that all the genotypes except two showed somaclonal variations .Among all ,the variation frequency of Jinliang No 5 was as high as 26%.The average frequency of all the investigated lines was 11.24%. Many somaclonal variants with special characters were obtained through variation screening method and one excellent hybrid named Jinza No 18 was developed and released.