Abstract: The paper described the new and traditional test methods for screening Brucellosis. The Brucellosis is an infectious disease, which cause not only economic loss but also the threat to the life of human and animal. The gene vaccination will be used for controlling it. The old detection technique has many deficiencies and will be replaced; the competitive ELISA has not diagnosing standards. The indirect ELISA can not separate the vaccine antibody and bacterial antibody even that were fast, accurate and advanced. Recently, the diagnostic technique has been constantly updated with molecular biology development, such as gene detection, fluorescence polarization assay and so on. PCR can test the 1pg of Brucella DNA, and the bacterial DNA test was most reliable. The Fluorescence Polarization Assay was accuracy and can be used for field work for livestock and wild animals.