Preliminary Study on Physiological Characteristics and Disease Control Effect of Huanglong Vaccine (HB-1) in Tomato Seedling Stage

doi:10.11924/j.issn.1000-6850.casb2020-0039

Chinese Agricultural Science Bulletin ›› 2021, Vol. 37 ›› Issue (7): 100-105.doi: 10.11924/j.issn.1000-6850.casb2020-0039

Special Issue: 生物技术；植物保护

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Preliminary Study on Physiological Characteristics and Disease Control Effect of Huanglong Vaccine (HB-1) in Tomato Seedling Stage

Li Xurui¹^,²(), Hu Xuefang¹^,², Sun Hao¹^,², Pei Haisheng¹^,², Deng Zhongxian³, Cao Jingxia⁴, Wang Shikui¹^,²()

¹Academy of Agricultural Planning and Engineering, Beijing 100125
²Key Laboratory of Agro-Products Postharvest Handling, Ministry of Agriculture and Rural Affairs, Beijing 100121
³Science and Technology Committee of Liaoning Association of Retired-Professors, Shenyang 110000
⁴Agricultural and Rural Bureau of Mengla County, Xishuangbanna Dai Autonomous Prefecture, Yunnan Province, Xishuangbanna Yunnan 666100

Received:2020-04-26 Revised:2020-07-24 Online:2021-03-05 Published:2021-03-17
Contact: Wang Shikui E-mail:surralee@163.com;wosk1963@shou.com

Abstract

Abstract:

To clarify the effects of Huanglong vaccine (HB-1) on tomato physiological characteristics and growth, tomato virus disease and early blight, through laboratory biological active determination experiments, we determined the effects of different concentrations of HB-1 on tomato apparent traits, peroxidase (POD) activity and malondialdehyde (MDA) content in tomato leaf; through field experiments, the control efficiency of HB-1 on tomato virus disease and early blight was determined. The results of laboratory experiments showed that HB-1 diluted 1000-fold, 2000-fold, and 3000-fold solutions could significantly increase POD activity and reduce MDA content. Among them, compared with that of control, the POD activity was more than 7 times higher under 2000-fold treatment, and the disease resistance effect was extremely significant. HB-1 could significantly increase tomato seedling plant height, stem diameter and leaf number under the dilution of 1000-3000 folds, and promote tomato seedling growth. In field experiments, the HB-1 diluted 800-fold solution had a 96.40% and 70.30% control efficiency on tomato virus disease and early blight, respectively. It shows that HB-1 has a good control efficiency on tomato virus disease and early blight within the test dilution range, and could obviously promote the growth of tomato seedlings and increase POD activity. HB-1 is suitable for the prevention and treatment of tomato virus disease and early blight, and has certain development and promotion value.

Key words: Huanglong vaccine (HB-1), nanoscale iodine, tomato virus disease, tomato early blight, peroxidase (POD) activity, malondialdehyde (MDA) content

CLC Number:

S481+.9

Li Xurui, Hu Xuefang, Sun Hao, Pei Haisheng, Deng Zhongxian, Cao Jingxia, Wang Shikui. Preliminary Study on Physiological Characteristics and Disease Control Effect of Huanglong Vaccine (HB-1) in Tomato Seedling Stage[J]. Chinese Agricultural Science Bulletin, 2021, 37(7): 100-105.

Figures/Tables 4

References 28

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处理	番茄病毒病（斑萎、红茎）		番茄早疫病
处理	病情指数	防治效果/%	病情指数	防治效果/%
清水(CK)	26.62		19.25
800倍	0.95	96.40	5.72	70.30
1500倍	7.80	70.70	8.12	57.80
2500倍	12.00	54.90	9.85	48.80

处理	番茄病毒病（斑萎、红茎）		番茄早疫病
处理	病情指数	防治效果/%	病情指数	防治效果/%
清水(CK)	26.62		19.25
800倍	0.95	96.40	5.72	70.30
1500倍	7.80	70.70	8.12	57.80
2500倍	12.00	54.90	9.85	48.80

Preliminary Study on Physiological Characteristics and Disease Control Effect of Huanglong Vaccine (HB-1) in Tomato Seedling Stage

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