Abstract: Based on the hypothesis of DNA fragment hybridization, exogenous DNA can be introduced into plant genome by pollen-tube pathway, producing progenies with genetically varied traits. New cultivars with fine agronomic traits can be selected from these progenies. The exogenous DNA can be the gene of interest or the whole genomic DNA from donor plants that possess the trait of importance. Because this method is simple and easy to operate, and the receptor plants are not limited to any plant species or genotypes, more and more plant breeders are adopting this technology. Significant progress has been made in rice breeding using this method, and new rice cultivars with high resistance to plant diseases, high quality and high yield have been bred and widely adopted by farmers. This article is intended to introduce the methods for the direct introduction of the exogenous DNA into plant genome, the genetic variations of the progenies produced and the factors that affect the effectiveness of the pollen-tube pathway. The merits, problems and the prospect for the pollen-tube pathway will also be discussed.