Abstract: In order to introduce techniques of flue-cured tobacco seedling production with floating system in Hunan Province, experiments were carried out on formulation of medium and nutrient solution as well as other suitable tobacco seedling production techniques. The results suggest that medium 14 mixing of peat, fine perlite, and carbonized chaff to a scale is recommended to tobacco seedling production with floating system. It indicates that because of the utilization of mineral nutrition, such as Ca2+ and Mg2+, in water, nutrient solution 2, which is suitable to tobacco seedling growth and development, is cheaper in costs of production. It shows that the middle ten days of December are the most suitable sowing date, witch guarantee flue-cured tobacco more than 120 days growth duration after transplanting and avoid conflicts between tobacco production and succession crop production. It indicates that 3~5 times of leaf cutting and 1/3~1/2 cutting area of tobacco leaf are in favor of high quality of tobacco seedling. Demonstration tests show that tobacco seedling production with floating system gains an advantage over two-stage system in cost of tobacco seedling production, seedling quality, prevention and cure of tobacco disease as well as production trait, etc