QTL Mapping and Candidate Gene Analysis for Bolting and Flowering Time of Flowering Chinese Cabbage

doi:10.11924/j.issn.1000-6850.casb2022-0330

Abstract

Abstract:

Bolting and flowering days are the two critical quantitative traits of flowering Chinese cabbage, at the same time, these traits are also affected by various environmental factors (temperature, light, soil, hormones, etc.), so clarifying the molecular genetic mechanism of the bolting and flowering process of flowering Chinese cabbage is quite difficult. In this experiment, two plant materials with significant differences in bolting and flowering days were selected as parents for crossbreeding. A total of 150 lines were selected from the F₂population for genetic map construction. The map constructed contained 4253 loci, representing 10940 single nucleotide polymorphism (SNP) markers spanning 1030.04 centi Morgans (cM) over 10 linkage groups (LGs). A total of 4 QTLs for bolting time and flowering time were obtained. Mixed linear composite interval mapping method was used to conduct QTL analysis for bolting and flowering traits of flowering Chinese cabbage, and bioinformatics was applied to identify some candidate genes. Three candidate genes (Bra004125, Bra004162 and Bra004165) were identified in the main QTL regions, which could control the formation of floral organs and interact with multiple signaling pathways that induce early flowering in plants.

Key words: flowering Chinese cabbage, genetic map, QTL mapping, bolting and flowering time, candidate gene analysis

LI Guihua, FU Mei, LUO Wenlong, LUO Shanwei, GUO Juxian. QTL Mapping and Candidate Gene Analysis for Bolting and Flowering Time of Flowering Chinese Cabbage[J]. Chinese Agricultural Science Bulletin, 2023, 39(13): 19-24.

Figures/Tables 10

References 16

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样品名称	SLAF数	总深度	平均测序深度
义农50天菜心	87833	3579100	40.75
湘红薹1号	80935	3188840	39.40
F₂平均	77079	762464	9.89

样品名称	SLAF数	总深度	平均测序深度
义农50天菜心	87833	3579100	40.75
湘红薹1号	80935	3188840	39.40
F₂平均	77079	762464	9.89

连锁群	标记数	图谱长度/cM	最大间距/cM	标记间隔/cM	标记完整度/%	双交换比例/%	缺失百分率/%
LG 1	525	87.17	5.02	0.17	99.81	0.00	0.15
LG 2	677	118.36	3.20	0.17	100.00	0.01	0.35
LG 3	552	117.65	2.40	0.21	100.00	0.00	0.59
LG 4	284	82.96	2.40	0.29	100.00	0.02	0.27
LG 5	235	140.03	14.99	0.60	99.15	0.01	0.68
LG 6	411	109.65	3.47	0.27	100.00	0.04	0.33
LG 7	342	102.89	6.38	0.30	99.71	0.03	0.21
LG 8	354	73.12	3.62	0.21	100.00	0.01	0.28
LG 9	491	120.92	4.28	0.25	100.00	0.01	0.33
LG 10	382	77.29	3.67	0.20	100.00	0.04	0.31
总和或平均	4253	1030.04	14.99	0.27	99.87

连锁群	标记数	图谱长度/cM	最大间距/cM	标记间隔/cM	标记完整度/%	双交换比例/%	缺失百分率/%
LG 1	525	87.17	5.02	0.17	99.81	0.00	0.15
LG 2	677	118.36	3.20	0.17	100.00	0.01	0.35
LG 3	552	117.65	2.40	0.21	100.00	0.00	0.59
LG 4	284	82.96	2.40	0.29	100.00	0.02	0.27
LG 5	235	140.03	14.99	0.60	99.15	0.01	0.68
LG 6	411	109.65	3.47	0.27	100.00	0.04	0.33
LG 7	342	102.89	6.38	0.30	99.71	0.03	0.21
LG 8	354	73.12	3.62	0.21	100.00	0.01	0.28
LG 9	491	120.92	4.28	0.25	100.00	0.01	0.33
LG 10	382	77.29	3.67	0.20	100.00	0.04	0.31
总和或平均	4253	1030.04	14.99	0.27	99.87

性状	P₁平均值	P₂平均值	F₁平均值	F₂变异范围
抽薹时间	52.0	34.0	38.0	32.0~52.0
开花时间	56.0	38.0	42.0	35.0~55.0