Abstract: Plantlets in vitro of ‘Domingo’, one of the red-flowering carnation breeds, were subcultured in vitro. They were selected for obtaining a clone, whose vitrification rate could be controlled less than 10%, by way of progressive subculture. After 27 times of subculture, such a clone was obtained. After 10 times of multiplying culture, the clone could keep the traits of its original one, and no harmful variation was observed, when they grew in a field.