Effect of Dimethylsulfoxide on Improving the Absorption and the Palatability of Quinestrol

doi:10.11924/j.issn.1000-6850.casb2023-0085

Abstract

Abstract:

In order to improve the utilization of quinestrol in baits and the palatability of quinestrol baits to rats, efficient and safe pesticide adjuvants were screened and the bait preparation method was optimized. Taking Rattus losea as the research object, and single-feed method was employed to investigate the effects of the solvent dimethylsulfoxide (DMSO) on the contraceptive effect and palatability of quinestrol drug bait. High-performance liquid chromatography was used to detect the content of quinestrol in brown rice in the different formulations of baits. The results showed that the content of quinestrol in rice grains of DMSO-quinestrol bait was 1.5 times higher than that in rice grains of quinestrol bait by high performance liquid chromatography. DMSO could increase the permeability of quinestrol. In addition, the amount of DMSO-quinestrol baits consumed by male and female Rattus losea were significantly lower than that of blank bait, the intake of quinestrol bait gradually decreased over time, and the third intake of quinestrol bait by male mice decreased by 37.3% compared to blank bait. The first intake of DMSO-quinestrol was only 24.1% of intake of quinestrol bait, and the digestion rate of blank bait was significantly higher than that of DMSO-quinestrol bait by 24.6% in female Rattus losea. Its body weight decreased significantly. The study clarifies that DMSO can achieve rapid and efficient dissolution of quinestrol and enhance its penetration in rough rice, thus realize the demand of pesticide and chemical fertilizer reduction and efficiency, DMSO is expected to be developed as a sterile agent formulation with good application prospect.

Key words: palatability, quinestrol, Rattus losea, dimethylsulfoxide

QIN Jiao, LIU Quansheng. Effect of Dimethylsulfoxide on Improving the Absorption and the Palatability of Quinestrol[J]. Chinese Agricultural Science Bulletin, 2023, 39(23): 82-87.

Figures/Tables 4

References 39

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项目	炔雌醚药饵		DMSO-炔药饵
项目	糙米	稻壳	糙米	稻壳
干重/g	7.85±0.02	2.15±0.02	7.82±0.05	2.18±0.05
峰面积	46.86±2.42	489.16±8.81	68.62±1.09	445.91±4.56
浓度/(μg/mL)	8.89±0.43	87.70±1.57	12.77±0.20	80.00±0.81
样品炔雌醚含量/(mg/g)	0.04±0.00	0.44±0.01	0.06±0.00	0.40±0.01

项目	炔雌醚药饵		DMSO-炔药饵
项目	糙米	稻壳	糙米	稻壳
干重/g	7.85±0.02	2.15±0.02	7.82±0.05	2.18±0.05
峰面积	46.86±2.42	489.16±8.81	68.62±1.09	445.91±4.56
浓度/(μg/mL)	8.89±0.43	87.70±1.57	12.77±0.20	80.00±0.81
样品炔雌醚含量/(mg/g)	0.04±0.00	0.44±0.01	0.06±0.00	0.40±0.01

体重/g	雄鼠			雌鼠
体重/g	c	t₁	t₂	c	t₁	t₂
第1次	-3.03±1.37^a	-1.77±1.25^a	-16.55±1.36^b	-7.64±1.77^ab	-4.49±1.74^a	-12.24±1.11^b
第2次	2.07±1.39^a	-2.87±1.75^ab	-7.87±2.35^b	-3.18±2.42^a	-4.07±1.06^a	-12.19±0.97^b
第3次	-0.18±1.70^a	-4.08±2.07^ab	-6.73±1.73^b	-1.35±2.03^a	-1.31±1.18^a	-10.54±1.78^b

体重/g	雄鼠			雌鼠
体重/g	c	t₁	t₂	c	t₁	t₂
第1次	-3.03±1.37^a	-1.77±1.25^a	-16.55±1.36^b	-7.64±1.77^ab	-4.49±1.74^a	-12.24±1.11^b
第2次	2.07±1.39^a	-2.87±1.75^ab	-7.87±2.35^b	-3.18±2.42^a	-4.07±1.06^a	-12.19±0.97^b
第3次	-0.18±1.70^a	-4.08±2.07^ab	-6.73±1.73^b	-1.35±2.03^a	-1.31±1.18^a	-10.54±1.78^b