Abstract: To gain cultivation model of high yield of transgenic hybrid cotton，the relationship between transgenic hybrid cotton yield and plant density, N, K, DPS, was studied by means of quaternary quadratic regression rotational combination design. The relations between the main effect of yield and interaction of four synthetic measures of agriculture namely density, nitrogenous fertilizer, potassium fertilizer, DPS have been analyzed. Their effect to F1 yield is arranged from big to small density, DPS, nitrogenous fertilizer, potassium fertilizer; F2 is arranged from big to small potassium fertilizer, nitrogenous fertilizer, density, DPS. the best mathematics regression models of cultural factors on yield and component: F1 is density 21500 plant/hm2, nitrogenous fertilizer 450kg/hm2, potassium fertilizer 450kg/hm2, DPS75g/hm2 can obtained topmost lint yield 1556kg/hm2. F2 density 15000 plant /hm2, nitrogenous fertilizer 750kg/hm2, potassium fertilizer 300kg/hm2, DPS135g/hm2 an obtained topmost lint yield 1219.35kg/hm2.