Genome-wide Identification and Bioinformatic Analysis of ENOD93 Gene Family in Rice

doi:10.11924/j.issn.1000-6850.casb2021-0624

Abstract

Abstract:

The aim of this study was to provide reference for the functional study of early nodulation protein ENOD93 in non-legumes. In this study, bioinformatics method was used to identify rice ENOD93 gene family. The physical and chemical properties, chromosome location, gene structure, protein structure, expression spectrum and evolutionary relationship of the members were analyzed. The results showed that there were 7 members of rice ENOD93 gene family, which were distributed on chromosomes 2 and 6, and the gene structure was relatively simple. Moreover, most ENOD93 genes are highly similar in the distribution and arrangement of conserved domain and motif. The results of RNA-Seq data analysis showed that ENOD93 gene family was highly expressed in pistils, seeds and embryos, and the expression level of some gene was induced by stress. Based on the phylogenetic analysis of nine monocotyledonous and dicotyledonous species, 31 ENOD93 gene family members were divided into four distinct groups. The expression of ENOD93 gene in rice was different in different tissues and at different developmental stages, and some genes were induced by stress, suggesting that ENOD93gene family was involved in the development process of many plant tissues and played an important role in the response to stress.

Key words: rice, ENOD93 gene family, gene identification, bioinformatics, adversity stress

LI Guzi, LIU Qun’en, CHEN Daibo, YU Ping. Genome-wide Identification and Bioinformatic Analysis of ENOD93 Gene Family in Rice[J]. Chinese Agricultural Science Bulletin, 2023, 39(27): 95-102.

Figures/Tables 9

References 25

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基因名称	基因ID	氨基酸数量	分子量/kD	等电点	总平均疏水指数	不稳定指数	脂溶指数	信号肽预测
OsENOD93-1	LOC_Os06g05010	116	12.42	10.95	0.020	36.96	85.26	NA
OsENOD93-2	LOC_Os06g04940	139	14.90	9.98	0.282	46.20	87.99	NA
OsENOD93-3	LOC_Os06g04950	115	12.23	10.29	0.060	33.10	83.48	NA
OsENOD93-4	LOC_Os06g04990	115	12.31	10.95	0.055	38.26	86.87	NA
OsENOD93-5	LOC_Os06g05000	115	12.17	10.35	0.081	27.63	83.48	NA
OsENOD93-6	LOC_Os06g05020	115	12.28	10.95	0.077	39.00	87.74	NA
OsENOD93-7	LOC_Os02g13380	154	16.43	11.42	-0.266	67.09	81.88	NA

基因名称	基因ID	氨基酸数量	分子量/kD	等电点	总平均疏水指数	不稳定指数	脂溶指数	信号肽预测
OsENOD93-1	LOC_Os06g05010	116	12.42	10.95	0.020	36.96	85.26	NA
OsENOD93-2	LOC_Os06g04940	139	14.90	9.98	0.282	46.20	87.99	NA
OsENOD93-3	LOC_Os06g04950	115	12.23	10.29	0.060	33.10	83.48	NA
OsENOD93-4	LOC_Os06g04990	115	12.31	10.95	0.055	38.26	86.87	NA
OsENOD93-5	LOC_Os06g05000	115	12.17	10.35	0.081	27.63	83.48	NA
OsENOD93-6	LOC_Os06g05020	115	12.28	10.95	0.077	39.00	87.74	NA
OsENOD93-7	LOC_Os02g13380	154	16.43	11.42	-0.266	67.09	81.88	NA