Non-Specific Effects of Apricot Shell Wood Vinegar Solution on Henosepilachna vigintioctopunctata

doi:10.11924/j.issn.1000-6850.casb2023-0387

Abstract

Abstract:

To investigate the growth inhibition, anti-feeding and repellent activity of apricot shell wood vinegar on the Henosepilachna vigintioctopunctata, to clarify the non-specific effect of apricot shell wood vinegar on the Mots, and to provide a theoretical basis for the application of the Mots’ green prevention and control and the application of wood vinegar for pest control. The Mots were fed with leaves of Solanum nigrum treated with different concentrations of apricot shell wood vinegar solution, and the body weight gain of 4^th instar larvae, non-selective anti-feeding and selective anti-feeding of adults and larvae as well as behavioral choices of different concentrations of apricot shell wood vinegar solution were measured in each period, and the effects of different concentrations of apricot shell wood vinegar solution on the weight gain inhibition, anti-feeding and repellency were analyzed, respectively. The results showed that, the weight gain of 4^th instar larvae, non-selective anti-feeding and selective anti-feeding of adults and larvae, and behavioral choice were all significantly affected by apricot shell wood vinegar solution, among which the weight gain inhibition of 4^th instar larvae by apricot shell wood vinegar solution reached 83% at 48 h. The highest non-selective anti-feeding rates were 92% and 82% for 4^th instar larvae and adults, respectively; the highest selective anti-feeding rates were 87% and 78% for 4^th instar larvae and adults, respectively; the Mots larvae and adults in the four-quadrant olfactometer showed highly significant repellent effect, with the highest repellent index of 0.67 for larvae and 0.95 for adults. It’s shown that apricot shell wood vinegar had significant weight gain inhibition, anti-feeding and repellent activity on the Mots. The concentration of apricot shell wood vinegar also had significant non-specific effects on the Mots.

Key words: Henosepilachna vigintioctopunctata, wood vinegar, growth inhibition, anti-feeding effect, repellent effect, four-quadrant olfactometer

GAN Yangguang, YE Lefu, LI Zhenyu, WANG Liqing, CHI Yang, XU Zhen, FU Xue. Non-Specific Effects of Apricot Shell Wood Vinegar Solution on Henosepilachna vigintioctopunctata[J]. Chinese Agricultural Science Bulletin, 2024, 40(13): 121-128.

Figures/Tables 10

References 27

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浓度/(mL/L)	24 h		48 h
浓度/(mL/L)	取食面积/cm²	非选择性拒食/%	取食面积/cm²	非选择性拒食/%
CK	3.02±0.28a	0d	5.87±0.45a	0d
1	2.15±0.21b	28.89±7.05c	4.06±0.39b	30.89±6.67c
2	1.63±0.15c	45.78±5.92b	2.94±0.41c	49.95±6.88b
5	1.32±0.16c	56.18±5.19b	2.32±0.34c	60.53±5.74b
10	0.66±0.08d	78.11±2.76a	1.30±0.17d	77.85±2.84a
20	0.43±0.12d	85.65±3.84a	0.71±0.13d	87.87±2.20a
50	0.42±0.10d	86.06±3.38a	0.79±0.22d	86.58±3.71a
100	0.28±0.06d	90.62±1.94a	0.48±0.09d	91.90±1.61a
回归方程	y=0.9432x+4.5908		y=0.9326x+4.6690
拒食中浓度AFC₅₀/(mL/L)	2.72		2.26
R²	0.9370		0.9371

浓度/(mL/L)	24 h		48 h
浓度/(mL/L)	取食面积/cm²	非选择性拒食/%	取食面积/cm²	非选择性拒食/%
CK	3.02±0.28a	0d	5.87±0.45a	0d
1	2.15±0.21b	28.89±7.05c	4.06±0.39b	30.89±6.67c
2	1.63±0.15c	45.78±5.92b	2.94±0.41c	49.95±6.88b
5	1.32±0.16c	56.18±5.19b	2.32±0.34c	60.53±5.74b
10	0.66±0.08d	78.11±2.76a	1.30±0.17d	77.85±2.84a
20	0.43±0.12d	85.65±3.84a	0.71±0.13d	87.87±2.20a
50	0.42±0.10d	86.06±3.38a	0.79±0.22d	86.58±3.71a
100	0.28±0.06d	90.62±1.94a	0.48±0.09d	91.90±1.61a
回归方程	y=0.9432x+4.5908		y=0.9326x+4.6690
拒食中浓度AFC₅₀/(mL/L)	2.72		2.26
R²	0.9370		0.9371

浓度/(mL/L)	24 h		48 h
浓度/(mL/L)	取食面积/cm²	非选择性拒食/%	取食面积/cm²	非选择性拒食/%
CK	8.09±0.38a	0f	15.02±0.59a	0e
1	5.64±0.33b	30.30±4.08e	10.17±0.45b	32.28±3.05d
2	5.33±0.20b	34.16±2.52e	9.54±0.38b	36.47±2.56d
5	4.47±0.30c	44.75±3.66d	7.71±0.58c	48.70±3.87c
10	2.94±0.22d	63.60±2.76c	5.10±0.41d	66.07±2.73b
20	2.61±0.18de	67.71±2.20bc	4.40±0.34d	70.73±2.27b
50	2.06±0.23ef	74.59±2.90ab	3.01±0.40e	79.93±2.65a
100	1.62±0.20f	80.02±2.50a	2.72±0.49e	81.91±2.65a
回归方程	y=0.7232x+4.4567		y=0.7509x+4.5166
拒食中浓度AFC₅₀/(mL/L)	5.64		4.4
R²	0.9694		0.9718

浓度/(mL/L)	24 h		48 h
浓度/(mL/L)	取食面积/cm²	非选择性拒食/%	取食面积/cm²	非选择性拒食/%
CK	8.09±0.38a	0f	15.02±0.59a	0e
1	5.64±0.33b	30.30±4.08e	10.17±0.45b	32.28±3.05d
2	5.33±0.20b	34.16±2.52e	9.54±0.38b	36.47±2.56d
5	4.47±0.30c	44.75±3.66d	7.71±0.58c	48.70±3.87c
10	2.94±0.22d	63.60±2.76c	5.10±0.41d	66.07±2.73b
20	2.61±0.18de	67.71±2.20bc	4.40±0.34d	70.73±2.27b
50	2.06±0.23ef	74.59±2.90ab	3.01±0.40e	79.93±2.65a
100	1.62±0.20f	80.02±2.50a	2.72±0.49e	81.91±2.65a
回归方程	y=0.7232x+4.4567		y=0.7509x+4.5166
拒食中浓度AFC₅₀/(mL/L)	5.64		4.4
R²	0.9694		0.9718

浓度/(mL/L)	处理	24 h			48 h
浓度/(mL/L)	处理	取食面积/cm²		选择性拒食率/%	取食面积/cm²		选择性拒食率/%
1	C	3.30±0.25^**		35.75±5.26e	6.16±0.38^**		34.51±2.73e
1	T	1.56±0.16		35.75±5.26e	2.99±0.19		34.51±2.73e
2	C	2.92±0.21^**		39.38±5.06de	4.88±0.30^**		38.85±4.53e
2	T	1.30±0.17		39.38±5.06de	2.19±0.22		38.85±4.53e
5	C	2.44±0.25^**		49.84±4.31d	3.91±0.24^**		54.00±2.55d
5	T	0.83±0.12		49.84±4.31d	1.18±0.11		54.00±2.55d
10	C	2.25±0.24^**		63.97±3.46c	3.22±0.26^**		67.54±2.05c
10	T	0.50±0.09		63.97±3.46c	0.64±0.09		67.54±2.05c
20	C	1.53±0.23^**		72.86±2.92bc	2.66±0.27^**		77.22±2.20b
20	T	0.24±0.04		72.86±2.92bc	0.36±0.06		77.22±2.20b
50	C	1.54±0.17^**		83.33±1.72ab	2.66±0.27^**		83.40±2.01ab
50	T	0.15±0.03		83.33±1.72ab	0.25±0.05		83.40±2.01ab
100	C	1.54±0.15^**		85.26±1.55a	2.52±0.28^**		86.56±2.20a
100	T	0.12±0.01		85.26±1.55a	0.17±0.02		86.56±2.20a
回归方程			y=0.7794x+4.5558			y=0.8251x+4.5458
拒食中浓AFC₅₀/(mL/L)			3.72			3.55
R²			0.9821			0.9751