Study on Traits of First Generation (SP1) Soybean Seed Induced by Space Mutagenesis

doi:10.11924/j.issn.1000-6850.casb2025-0657

Abstract

Abstract:

This study investigates the effects of space mutagenesis on physiological indicators and antioxidant enzymes of different soybean varieties, providing foundational materials for soybean germplasm improvement and variety selection, and providing a foundation and reference for the deepening of soybean space mutagenesis breeding and the application of this technology in the genetic improvement of leguminous crops. Taking the four superior soybean seeds carried by the "Shijian-19" satellite as the research objects, after being subjected to space mutagenesistion mutation treatment and returned to the ground for SP₁ generation ground cultivation, the soybean seeds that were not carried aboard served as the control group (CK). The growth traits, photosynthetic indicators, antioxidant enzymes, and soluble protein contents of these soybeans were measured and analyzed. The results showed that the germination rate of 'Deshun 34' and 'Dongchuang 19' was respectively increased by 13.2% and 17.6% compared with the CK, the germination potential of 'Dongchuang 19' was 9.4% higher than that of the CK; the leaf length of 'Deshun 14' was reduced by 8.9% compared with the CK, and the leaf width of 'Dongnong 219' was 28.0% lower than that of the CK; the plant height of 'Deshun 34' was 5.19% higher than that of the CK, and the plant width of 'Deshun 34', 'Deshun 14' and 'Dongchuang 19' was respectively reduced by 53.2%, 54.7% and 56.3%, the internode length of 'Dongchuang 19' was increased by 10.8% compared with the CK, and the branch number of 'Dongnong 219' was 28.0% lower than that of the CK; the single plant pod number of 'Dongnong 219' was increased by 7.7% compared with the CK. The net photosynthetic rate of 'Deshun 14' was increased by 17.3% compared with the CK. The POD activity of 'Deshun 34' and 'Deshun 14' was both increased compared with the CK; the MDA content of 'Deshun 14' was highly significantly higher than that of the CK; the soluble protein content of 'Dongchuang 19' was highly significantly increased compared with the CK. In conclusion, the traits of the soybean plants after space mutagenesis showed varying degrees of variation. Among them, the effect on the plant width of soybeans was the most significant, and the photosynthetic indicators of soybeans improved to a certain extent; the changes in POD activity, MDA and soluble protein content were relatively large, while the change in SOD was the smallest, and the variation was different among different varieties.

Key words: soybean, space mutagenesis, variety, growth trait, SOD, POD, MDA, soluble protein

FANG Yi, ZHU Baoqing, WANG Tongxin, Li Fuxue, FENG Jianhang, YANG Siyi, YU Xudong. Study on Traits of First Generation (SP₁) Soybean Seed Induced by Space Mutagenesis[J]. Chinese Agricultural Science Bulletin, 2025, 41(36): 35-42.

Figures/Tables 10

References 23

[1]	官梅. 作物航天育种研究进展[J]. 作物研究, 2009, 23(5):298-305.
[2]	潘光辉, 尹贤贵, 杨琦凤, 等. 作物航天诱变育种研究进展[J]. 西南农业学报, 2005, 18(6):853-857.
[3]	刘录祥, 郭会君, 赵林姝, 等. 我国作物航天育种20年的基本成就与展望[J]. 核农学报, 2007(6):589-592.
[4]	刘鑫磊, 马岩松, 栾晓燕, 等. 航天诱变对大豆品种光合性状的影响[J]. 大豆科学, 2011, 30(4):606-608.
[5]	吕爽, 郑伟, 郭泰, 等. 大豆航天搭载与60Co-γ辐射复合诱变效果分析[J]. 中国种业, 2012(2):34-36.
[6]	张勇, 杨兴勇, 董全中, 等. 利用空间诱变技术选育大豆新品种克山1号[J]. 核农学报, 2013, 27(9):1241-1246. doi: 10.11869/hnxb.2013.09.1241
[7]	赵曦, 王广金, 李铁, 等. 大豆空间诱变突变体的RAPD分析[J]. 核农学报, 2014, 28(5):772-776. doi: 10.11869/j.issn.100-8551.2014.05.0772
[8]	于绍轩, 韩粉霞, 孙君明, 等. 空间环境对大豆主要农艺性状及蛋白品质的诱变效应[J]. 核农学报, 2010, 24(3):453-459. doi: 10.11869/hnxb.2010.03.0453
[9]	郑伟, 郭泰, 王志新, 等. 大豆航天育种研究进展[J]. 辐射研究与辐射工艺学报, 2015, 33(5):3-11.
[10]	宋兴舜, 吴迪, 刘雪梅, 等. 大青杨航天诱变植株早期抗氧化酶生化指标测定[J]. 林业科学, 2009, 45(7):145-149.
[11]	王朔楠, 孙静, 郭嘉莹, 等. 种子发芽指标及其测算方法[J]. 麦类作物学报, 2023, 43(2):190-196.
[12]	谢松青, 赵阳, 王颖. 种子发芽势对作物田间出苗率的重要性分析[J]. 现代农村科技, 2019(4):45.
[13]	何陈林, 拉喜那木吉拉, 红艳, 等. 北苍术种子萌发特性比较研究[J]. 智慧农业导刊, 2022, 2(22):23-25,28.
[14]	于国栋, 李莲芳, 孙昂, 等. 微波辐射和IBA浸种对云南松种子发芽的影响[J]. 种子, 2014, 33(5):8-12.
[15]	张玉, 白史且, 王曾珍, 等. 航天搭载菊苣种子的生物学特性研究[J]. 草业学报, 2012, 21(2):300-304.
[16]	李洁, 马甜甜, 剡转转, 等. 12C6+辐照及航天诱变对无芒隐子草种子萌发、幼苗生长及叶绿素荧光特性的影响[J]. 草业科学, 2019, 36(8):2033-2041.
[17]	彭依晴. 航天诱变柴胡SP2代优良株系田间筛选及其综合评价研究[D]. 咸阳: 西北农林科技大学, 2023.
[18]	王建丽, 申忠宝, 潘多锋, 等. 航天搭载紫羊茅SP2代农艺性状诱变效应研究[J]. 北方园艺, 2015(1):63-67.
[19]	刘自学, 郑群英, 汪玺. 6种草坪草叶片的气孔特征与气孔传导力[J]. 草业科学, 2005, 22(8):71-75.
[20]	王菲, 尹伟伦, 夏新莉, 等. 空间条件对高羊茅SP1代形态及光合生理特性的诱变效应[J]. 北京林业大学学报, 2010, 32(3):106-111.
[21]	贺鹏, 朱列书, 钟波. 航天诱变对烤烟大田期SOD活性的影响[J]. 中国农学通报, 2008, 24(7):123-125.
[22]	林善枝, 李雪平, 张志毅. 低温锻炼对毛白杨幼苗抗冻性和总可溶性蛋白质的影响[J]. 林业科学, 2002(6):137-141.
[23]	吴岳轩, 曾富华. 空间飞行对番茄种子活力及其活性氧代谢的影响[J]. 园艺学报, 1998, 25(2):165-169.

品种	发芽率/%			发芽势/%
品种	地面对照(CK)	航天诱变(SP₁)	比CK±	地面对照(CK)	航天诱变(SP₁)	比CK±
德顺34	50.0±1.9	63.2±2.1^*	+13.2%	23.0±1.1	26.2±1.3	+3.2%
德顺14	90.0±2.4	73.4±2.3^*	-16.6%	43.6±1.5	32.2±1.8^*	-11.4%
东创19	63.2±1.7	80.8±2.6^*	+17.6%	48.0±1.4	57.4±2.1^*	+9.4%
东农219	82.0±2.3	86.6±2.2	+4.6%	56.8±1.2	60.2±2.0	+3.4%

品种	发芽率/%			发芽势/%
品种	地面对照(CK)	航天诱变(SP₁)	比CK±	地面对照(CK)	航天诱变(SP₁)	比CK±
德顺34	50.0±1.9	63.2±2.1^*	+13.2%	23.0±1.1	26.2±1.3	+3.2%
德顺14	90.0±2.4	73.4±2.3^*	-16.6%	43.6±1.5	32.2±1.8^*	-11.4%
东创19	63.2±1.7	80.8±2.6^*	+17.6%	48.0±1.4	57.4±2.1^*	+9.4%
东农219	82.0±2.3	86.6±2.2	+4.6%	56.8±1.2	60.2±2.0	+3.4%

品种	叶长/cm			叶宽/cm
品种	地面对照(CK)	航天诱变(SP₁)	比CK±	地面对照(CK)	航天诱变(SP₁)	比CK±
德顺34	5.8±0.38	5.77±0.82	-0.5%	3.31±0.38	3.14±0.44^*	-5.1%
德顺14	7.39±0.54	6.73±0.76^**	-8.9%	4.31±0.74	3.81±0.44^*	-11.6%
东创19	7.64±0.55	7.06±0.51^*	-7.6%	3.61±0.42	3.38±0.36^*	-6.4%
东农219	6.35±0.75	6.03±0.84^*	-5.0%	4.57±0.54	3.29±0.57^**	-28.0%

品种	叶长/cm			叶宽/cm
品种	地面对照(CK)	航天诱变(SP₁)	比CK±	地面对照(CK)	航天诱变(SP₁)	比CK±
德顺34	5.8±0.38	5.77±0.82	-0.5%	3.31±0.38	3.14±0.44^*	-5.1%
德顺14	7.39±0.54	6.73±0.76^**	-8.9%	4.31±0.74	3.81±0.44^*	-11.6%
东创19	7.64±0.55	7.06±0.51^*	-7.6%	3.61±0.42	3.38±0.36^*	-6.4%
东农219	6.35±0.75	6.03±0.84^*	-5.0%	4.57±0.54	3.29±0.57^**	-28.0%

品种	株高/cm			茎粗/mm
品种	地面对照(CK)	航天诱变(SP₁)	比CK±	地面对照(CK)	航天诱变(SP₁)	比CK±
德顺34	22.54±2.88	23.71±2.86^*	+5.19%	1.9±0.36	1.7±0.44^*	-10.53%
德顺14	20.32±2.68	19.42±1.91^*	-4.43%	3.8±0.43	3.3±0.52^*	-13.16%
东创19	21.75±2.72	20.00±3.40^*	-8.05%	4.3±0.51	3.9±0.84^*	-9.30%
东农219	15.35±2.19	15.46±2.53	+0.72%	4.0±0.73	3.7±0.86^*	-7.50%

Study on Traits of First Generation (SP₁) Soybean Seed Induced by Space Mutagenesis

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 23

Related Articles 15

Recommended Articles

Metrics

Comments

For authors

For reviewers

Reader center

Contact us

品种	净光合速率/[µmol/(m²·s)]			气孔导度/[mol/(m²·s)]
品种	地面对照(CK)	航天诱变(SP1)	比CK±	地面对照(CK)	航天诱变(SP1)	比CK±
德顺34	20.56±4.38	22.49±2.30^*	+9.4%	1.16±0.04	1.29±0.31^*	+11.2%
德顺14	20.59±4.20	24.16±1.50^**	+17.3%	1.04±0.17	1.32±0.26^**	+26.9%
东创19	19.66±3.12	20.86±1.49^*	+6.1%	0.65±0.21	0.91±0.15^**	+40.0%
东农219	22.18±6.10	23.73±2.13^*	+6.9%	1.14±0.50	1.27±0.24^*	+11.4%

[1]	JIANG Zijian, YANG Maolin, YANG Xiaoxu, LIU Chang, LIU Dajun, FENG Guojun. Effects of Abscisic Acid on Growth and Cold Resistance of Phaseolus vulgaris Seedlings Under Low Temperature Stress [J]. Chinese Agricultural Science Bulletin, 2025, 41(9): 38-46.
[2]	WU Suxia, ZHAO Peng, FAN Hairong, SONG Shitao, CHEN Lina, JIN Changmin, CUI Yue. Effect of Bio-organic Fertilizer on Growth Trait of Millet [J]. Chinese Agricultural Science Bulletin, 2025, 41(8): 19-24.
[3]	LI Lihua, XUE Bingjie, WANG Yale, LI Xuejun, SUN Huan, SUN Jiping. Effects of Drought-rehydration on Physiological Indexes and Drought-related Gene Expression of ‘YY021’ Seedlings [J]. Chinese Agricultural Science Bulletin, 2025, 41(8): 57-62.
[4]	LIU Jiao, HU Xing, HUANG Xiaoxia, LIU Wenge, HE Mingxiong, LU Changyou, LUO Yigui. Planting Adaptability of New Flue-cured Tobacco Varieties ‘Yunyan 116’ and ‘Yunyan 300’ in Hechi Tobacco Region of Guangxi [J]. Chinese Agricultural Science Bulletin, 2025, 41(7): 22-28.
[5]	WANG Haoyi, QIAO Wei, LIN Yuying, YANG Haobo, JIE Weiguang. Research Status of Bacterial Degradation of Pesticide Residues in Soybean [J]. Chinese Agricultural Science Bulletin, 2025, 41(6): 126-131.
[6]	LI Xianghua, FAN Caiying, XU Qin, HOU Jian, WANG Hui, LIU Yanyan, WANG Heng, LIU Guangya, HAN Wei. Effects of Different Maize and Soybean Planting Patterns on Crop Yield, Economic Benefit and Soil Nutrient [J]. Chinese Agricultural Science Bulletin, 2025, 41(6): 22-28.
[7]	WANG Yangmei, LIU Yan, FENG Kui, DONG Hongping, LIU Shaowen, YANG Yong, ZHANG Rong, HE Lian. Relationship Between Agronomic Traits and Yield of Different Potato Varieties in Western Sichuan Plateau Region [J]. Chinese Agricultural Science Bulletin, 2025, 41(6): 29-37.
[8]	ZHU Yuanjie, TA Na, ZHAO Weiguo, ZHANG Mengru, LI Chengjun, HE Maochang. Seed Germination and Seedling Growth Physiological Characteristics of Brassica napus Under Simulated Drought Stress [J]. Chinese Agricultural Science Bulletin, 2025, 41(6): 44-52.
[9]	YAN Xiaohua, CAO Tian, ZHANG Guobin. Effect of Water Stress on Root Growth and Antioxidant Enzyme System of Cucumber Seedlings [J]. Chinese Agricultural Science Bulletin, 2025, 41(4): 31-38.
[10]	WANG Yanping, WANG Yaying, WANG Zhichun, QU Xuebin. Climatic Risk Zoning of Soybean Pod Borer Occurrence in Northeastern Inner Mongolia [J]. Chinese Agricultural Science Bulletin, 2025, 41(36): 142-148.
[11]	ZHANG Huaying, LIU Jiale, YANG Yang, XU Xintong, ZHANG Shuo, SHI Yingyuan, LIAO Wenjia, DONG Yuxin. Effects of Different Water Retaining Agents on Growth and Development of Soybean Seedlings Under Drought Stress [J]. Chinese Agricultural Science Bulletin, 2025, 41(36): 28-34.
[12]	YOU Si, JIANG Huyuan, YOU Wei, LIN Cong. Research on Climate Suitability for Soybean Cultivation in Hulunbuir Area from 1991 to 2022 [J]. Chinese Agricultural Science Bulletin, 2025, 41(36): 119-131.
[13]	SUN Haiwei, ZHANG Hong, REN Zhihong, WU Huanhuan, XIAO Wenmin, YANG Shengxiang. Quality Evaluation and Characteristics Analysis of Taishan Black Tea [J]. Chinese Agricultural Science Bulletin, 2025, 41(34): 137-146.
[14]	TUERHONG Gulinuer, TIAN Guo, YANG Qingchuan, YE Tingyu, YANG Minglu. Predatory Functional Response of Hippodamia variegata to Myzus persicae [J]. Chinese Agricultural Science Bulletin, 2025, 41(33): 139-145.
[15]	LEI Yuming, ZHENG Tianxiang, LI Huiwen, WANG Tingsan, WANG Aixia, GUAN Yuan. A New Silage Corn Cultivar ‘Jinhui 658’: Breeding and Characteristics Evaluation in Silk Road Arid and Cold Region [J]. Chinese Agricultural Science Bulletin, 2025, 41(33): 32-38.

品种	株幅/cm			底荚高/cm
品种	地面对照(CK)	航天诱变(SP₁)	比CK±	地面对照(CK)	航天诱变(SP₁)	比CK±
德顺34	22.24±1.78	10.4±0.73^**	-53.2%	8.1±0.32	7.6±0.36^*	-6.2%
德顺14	29.22±2.19	13.25±1.12^**	-54.7%	7.21±1.21	5.6±2.71^**	-22.3%
东创19	23.0±1.75	10.05±1.06^**	-56.3%	5.87±1.34	5.2±1.56^*	-11.4%
东农219	17.16±1.34	12.7±0.64^*	-26.0%	6.76±0.73	6.13±0.93^*	-9.3%

品种	株幅/cm			底荚高/cm
品种	地面对照(CK)	航天诱变(SP₁)	比CK±	地面对照(CK)	航天诱变(SP₁)	比CK±
德顺34	22.24±1.78	10.4±0.73^**	-53.2%	8.1±0.32	7.6±0.36^*	-6.2%
德顺14	29.22±2.19	13.25±1.12^**	-54.7%	7.21±1.21	5.6±2.71^**	-22.3%
东创19	23.0±1.75	10.05±1.06^**	-56.3%	5.87±1.34	5.2±1.56^*	-11.4%
东农219	17.16±1.34	12.7±0.64^*	-26.0%	6.76±0.73	6.13±0.93^*	-9.3%

品种	节间长/cm			分枝数/个
品种	地面对照(CK)	航天诱变(SP₁)	比CK±	地面对照(CK)	航天诱变(SP₁)	比CK±
德顺34	8.97±1.51	8.56±2.28^*	-4.6%	3.31±0.23	3.14±0.51^*	-5.1%
德顺14	8.6±2.02	7.73±1.50^**	-10.1%	4.31±0.21	3.81±0.26^*	-11.6%
东创19	7.9±2.07	8.75±0.49^**	+10.8%	3.61±0.30	3.38±0.32^*	-6.4%
东农219	6±0.72	5.97±0.73	-0.5%	4.57±0.15	3.29±0.24^**	-28.0%

品种	节间长/cm			分枝数/个
品种	地面对照(CK)	航天诱变(SP₁)	比CK±	地面对照(CK)	航天诱变(SP₁)	比CK±
德顺34	8.97±1.51	8.56±2.28^*	-4.6%	3.31±0.23	3.14±0.51^*	-5.1%
德顺14	8.6±2.02	7.73±1.50^**	-10.1%	4.31±0.21	3.81±0.26^*	-11.6%
东创19	7.9±2.07	8.75±0.49^**	+10.8%	3.61±0.30	3.38±0.32^*	-6.4%
东农219	6±0.72	5.97±0.73	-0.5%	4.57±0.15	3.29±0.24^**	-28.0%