Abstract: An Experiment of wide row and short space between plants (WRSSBP) planting methods of Mat Grass (Juncus effuses L) was carried out on paddy field developed by Quarternary Red Soil. The result showed that the optimum planting density with (WRSSBP) was 30 cm×15 cm, following by 25 cm×15 cm. The biomass yields were 13.20 t/hm2 and 12.76 t/hm2, and 23.5% and 19.3% higher significantly than normal planting method, respectively. The principle of increasing yield of WRSSBP were that exert margin advantage and harmonize development of individual and colony by decreasing space between plants and increasing row distance. The results of pen experiments showed that mid October was the optimum transplanting time and early-mid July was the optimum harvesting time.