Abstract: Quadratic regressive factorial experiment was used to optimize concentration of 6-BA and NAA in MS medium for shoot propagation from the stem segments of Polygonum multiflorum Thunb. The result showed that the most of shoot achieved by the 6-BA and NAA concentrations were in a rang of 1.26~1.99mg/L and 0.05~0.48mg/L respectively.The probability of the event was 95%.