Abstract: Rice matter production, distribution and yield of Xiushui79 were investigated by applying nitrogen at different growth stages with fertilizing 180 kg pure nitrogen per hectare during whole growth duration. The results as follows: The highest tillering number decreased with the decreasing proportion of applying nitrogen at earlier stage. Applying nitrogen at later stage could enhance the panicle formation rate and extend the developmental phase. Moreover, the yield with later nitrogen application was increased significantly compared with that of non- nitrogen. When nitrogen application with the ratio of basal fertilizer, tiller fertilizer, panicle fertilizer was 5:3:2, the yield amounted to maximum and it could increase by 3800.0kg/hm2 (that was 85.15%) than comparison. Nitrogen productivity decreased with the increasing basal fertilizer. Different proportion of applying nitrogen could effect considerably on the effective panicle number and the setting percentage, but had no significant effect on the number of filled grain and the thousand-grain weight. Therefore, maintaining sufficient spikelet number, appropriate later nitrogen application could extend the growth stage of flag leaves, prevent premature senescence and increase yield by enhancing setting percentage and thousand-grain weight.