Abstract: Purpose: to improve the solubility, emulsification and foaming ability of rice protein by enzymolysis. Methods: to select suitable way of applying enzyme by the enzymolysis curve, to optimize the enzymolysis condition with uniform design and to analysis data by Mathematica statistic software. Result: The efficiency of hydrolysis by Alcalase and Protamex together was better than that by single Alcalase or Protamex. The changes of solubility, emulsification and foaming activity of the hydrolysates were not same at the same hydrolytic condition. Conclusion: Using two proteases together could improve the solubility of rice protein more than using single protease. In the system of hydrolysis, Alcalase and Protamex exist cooperation with each other.