Abstract: In this paper, the browning and antibrowning in the tissue culture of Parthenocissus tricuspidata was studied. The materials are aseptic stem segments with cotyledons from Canada. The results showed that aseptic stem segments with cotyledons appreared more browning. Its browning rate reached even over 89.3%. Different concentrations of Vc or AC or PVP was added in medium, The results showed that VC is the best method on antibrowning and its suitable concentration is 1.5 g/L.