Abstract: 【OBJECTIVE】 In order to selected the herbicide of varieties and the use which has no phytotoxicity on Zhaodong Alfalfa, we investigated the herbicide resistance of the influence on Zhaodong Alfalfa. 【METHOD】 In this experiment, we observed and recorded the herbicide has effect on seeding conditions and grass conditions in the period of Alfalfa mycobacterium. 【RESULTS】 Several herbicide were cheesed which has no phytotoxicity on Alfalfa, there were 75% KUOYESAN spraying after seeding, 5% JINPUSHITE spraying after 2~4 leaves, 4% JINDOU spraying after 2~4 leaves, 10% LISHOU spraying after 2~4 leaves. The result showed 5%JINDOU has more effective than others. 【CONCLUSION】 The herbicide which has no phytotoxicity on Zhaodong Alfalfa was 5%JINDOU. The recommend method were spraying after seeding and after 2~4 leaves.