Detection of Sugarcane Ratoon Stunting Disease Pathogen with Polymerase Chain Reaction (PCR) and Nucleotide Sequence Analysis

Abstract

Abstract: PCR technique was established for detection of Leifsonia xyli subsp. xyli(Lxx.), the causal agent of sugarcane ratoon stunting disease(RSD), with specific primers based on the intergenic transcribed spacer(ITS) region of 16S-23S ribosomal DNA of Lxx. By sequence comparison of intergenic transcribed spacer (ITS) region of 16S-23S rDNA, it was revealed that RSD pathogen was highly conversant. Lxx guangdong isolate shares almost 100% nucleotide identity with Brazil, Australia and USA isolates. However, Lxx ITS sequences share only low identity with related close(Leifsonia xyli subsp. Cynodontis,Lxc) and morphologic similar bacteria(Clavibacter michiganensis subsp.michiganensis).

CLC Number:

S435.661

Shen Wankuan, Zhou Guohui, Deng Haihua, Zhou Linyun. Detection of Sugarcane Ratoon Stunting Disease Pathogen with Polymerase Chain Reaction (PCR) and Nucleotide Sequence Analysis[J]. Chinese Agricultural Science Bulletin, 2006, 22(12): 413-413.

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