Abstract: In this paper, three varieties of turfgrass, Cindy, Merit and Blue chip, were stressed under 5℃, -5℃ and -10℃, respectively, and their membrane permeability of the leaves were measured by conductivity, and the proline concentration of their leaves were determined by ninhydrin colorimetric method, aimed at searching law of membrane permeability and proline concentration change under chilling stress. The results showed that three kinds of low temperature exposure, the proline content in three varieties of turfgrass increased at significance level. As the turfgrass exposed under 5℃ and -10℃, the increase of proline content in Cindy was highest, and that in Merit and Blue chip were nearly equal. As the turfgrass stressed under -5℃, the increment of proline content in Blue chip was > in Cindy > in Merit, but no significant difference was in that of them. The proline increment under -10℃ was significantly less than that under -5℃, and was nearly equal to that under 5℃. The membrane permeability of the turfgrass also increased, which increment showed that of Cindy