Abstract: At first, the study has screened out N2 medium, the most suitable medium from five kinds of media. The medium has the advantage of preparing easily and economically, and comfort for massive application. The study has done experiment that the medium of N2 affect posterior seedling in test tube and growing about different kinds of seedling in test tube, and studied the key factor in the process of micro—potato application of tissue rapid propagation. The results of studies on Detoxified Technique of Potato: The best Culture Media of detoxified technique of Potato and the application of tissue rapid propagation of Micro-potato is N2: N1+IAA (5 mg/1000ml)+NAA (3 mg/1000ml).