Abstract: The promoter of a heat-inducible gene AtHSP70b was amplified from the genome DNA of Arabidopsis thaliana cv. Columbia, which had a length of 204bp and the same sequence as published previously. The expression vectors, pV-HSP70bGUS was constructed by inserting the promoter of AtHSP70b. The vector was introduced into Agrobacterium tumefaciens LBA4404 which was used to mediate the transfection of AtHSP70b promoter fragment into the tobacco (W38). The regenerated plants were analyzed and showed that the promoter fragment was integrated into the genome of tobacco. Histochemical staining of GUS activity of the transgenic plants proved that the promoter of AtHSP70b expressed unprecisely.