Abstract: The optimum conditions of extracting LPC from the fresh leaves of Ryegrass were studied by using acid-heat methods. And the amino acid contents of LPC were determined. The results show that the optimum extraction technique of LPC was as pH 4.0, the ratio 1:2 for fresh leaf to water and 70℃ with 5min. In such condition the protein concentration of LPC can reach 70.48%. The proportion of essential amino acid, sapor amino acid and drug-effective amino acid in total amino acid are 40.3247%, 22.2439% and 77.5609% separately. The LPC from ryegrass have nutritional, healthy and drug-effective value.