Abstract: Bar-transgenic wheat, Nong4- Bar gene transferred into D genome with the herbicide-resistant function, can be chosen as the parents to breed the varieties which can resist herbicide by the traditional way. In the process to insert Bar Gene, the effects on physiological and biochemical characteristics occurred because of the location of Bar Gene, which was studied at present time. But the effects on photosynthetic characteristics and yield trends are still under research. In this paper, different effects on trends of yield were found at first. And the photosynthetic characteristics were determined accordingly, which on the other hand showed the different effects on the photosynthetic characteristics of Bar-transgenic wheat and their hybrids. The results were as follows: Between Bar-transgenic wheat Nong4 and its hybrids, F1Nong4×Wanmai48 had the better photosynthetic characteristics and trends of yield than that of F1Wanmai48×Nong4. And mid-parent heterosis rate of F1Nong4×Wanmai48 was higher than that of F1Wanmai48×Nong4. For example ears of wheat, kenel weight super close advantage rate attain 5.17% and 4.41% respectively. So, according to the revelation, when the anti-herbicide hybrids were bred, Nong4-Bar gene into D genome was a better choice as father plant and high yield wheat was chosen as mother.