Abstract: With the leaf area of Gladiolus measured by digital camera and Photoshop software as a reference, the correction coefficient and regression equation were gained by calculating long diameters (L) and short diameters (W) of Gladiolus leaves. According to the method of leaf area correction coefficient, the equation for both red-peach and pink-rose were LA=0.65LW and LA=0.71LW, respectively. The regression equation of red-peach and pink-rose were LA=0.58×L×W+ 7.02 and LA=0.69×L×W+ 1.58, respectively. The result of variance analysis revealed that regression equation method could give a more reliable result than Leaf area correction coefficient method for the leaf area measurement.