An Efficient Method for Screening Mutants With Altered Responses to Iron Deficiency in Arabidopsis

Abstract

Abstract: Fe is an essential mineral nutrient for plant growth and development. Iron deficiency directly affects crop yield and quality. However, the molecular mechanism of iron nutrition is largely unknown. To understand how plant response to Fe deficiency stress, in this paper, we have developed an efficient method for genetic screening Arabidopsis mutants related to the stress based on the phenotypes in morphology and physiology. Using this screening method, we have isolated 11 putative T-DNA tagged mutants with alterations in sensitivity in response to iron deficiency. We have cloned one gene, which mutation caused increased sensitivity to Fe deficiency. Identification and characterization the determinants in Fe nutrition of plant will further our understanding on genetic basis of plant in response to nutrient stress. It will provide new insights on molecular improvement of crops.

CLC Number:

Q-33

Hu Haizhou, Sun Feifei, Li Bao, Wang Youning, Li Kexue, Jia Huizhen, Zhang Wensheng, Li Sishen, Li Xia. An Efficient Method for Screening Mutants With Altered Responses to Iron Deficiency in Arabidopsis[J]. Chinese Agricultural Science Bulletin, 2007, 23(6): 108-108.

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