Abstract: The purpose of this research is to induction and selection variants of Hypericum perforatum L. Using stem apexes of Hypericum perforatum as material, effects of Pingyangmycin (PYM) concentration and treating time as well as supplementary materials in medium on survival rate of stem apexes and influence of hormone combinations on induction rate of adventitious-buds from stem apexes were investigated. The results showed that the treatment of 80mg/L PYM for 20min was suitable for mutation. The survival rate of stem apexes could be improved by adding CW in medium and the combination of hormones 0.5mg/L BA with 0.01mg/L NAA was suitable for inducing normal adventitious buds and obtaining higher induction rate. The procedure for inducing and selecting variants from stem apexes of Hypericum perforatum was established, and a variant with higher hypericin was obtained.