Abstract: Three Cytoplasmic male sterile lines cultivated in Central-south Experiment Station, CNTC and 4 varieties of Flue-cured tobacco popularized in China were used to investigate the heterosis for quality characters, i.e., total sugars (TS), nicotine(Nic), total nitrogen (TN), chlorine(Cl), potassium oxide(K2O), TS/Nic, TN/Nic and K2O/Cl of lower leaves in Flue-cured tobacco. The results showed: (1) There were the heterosis from mid-parent value, Hm, performing in most crosses, and the positive significant crosses were accounted for 25.00% of crosses for TN, On the contrary, there were 33.33%、25.00% and 8.33%of negative significant crosses for TN/Nic, TS and K2O respectively. There were 41.67%, 16.67%, 8.33% and 8.33% of positive significant crosses and 8.33%, 8.33%, 33.33% and 8.33% of negative significant crosses for Nic, Cl, TS/Nic and K2O/Cl respectively(2) There were 25.00%, 16.67% and 8.33% of crosses performing the significant heterosis over high-parent, Hh, for TN, Nic and Cl respectively, and there were no crosses with significant Hh for the others (3) There was no cross performing the significant heterosis less lower-parent, Hl, for the quality trails except for Nic, TS/Nic and TN/Nic with 8.33%、16.67% and 8.33% of significant crosses respectively.