Abstract: To find the effects of sowing density and germinating conditions on the germination of flue-cured tobacco seed, the flue-cured tobacco seed inside germination was under an artificial climate chamber. The study on effects of sowing density, germinating conditions on the germination of male sterile Yunyan 87 (a flue-cured tobacco variety) pellet seed was conducted from 3 aspects ranging from sowing density, seedbed to watering. The results indicated that different sowing density posed greater effect on the germination of Yunyan 87’s pelleted seed than the germinating conditions did. When testing the seed germination, the suitable sowing density is 100 seeds per petri dish filled with 2 layers of filter paper soaked with tap water. Under these conditions, the germination force, the germination rate and the synchronization index of the pellet seed of Yunyan87 reached 76.0%，89.0% and 0.49 respectively.