Abstract: In order to explore the effect of N (NH4-N and NO3-N) and K interaction on the growth and nutrients uptake of rice, rice pot experiment was conducted. The results displayed that the amount of dry matter and nutrients taken up by rice with NO3-N application were larger than that with NH4-N application in the early growing period of rice (20 days of growth). But with the increase of growth period, the application of NH4-N was demonstrated to be much more beneficial to the growth and nutrients uptake of rice. In addition, the rate of K applied was perceived to have influence on the effect of N fertilizer application. The growth and nutrients uptake of rice could be largely lessened by the application of K (K2O) with a rate of 0.51 g/kg, when the N rate was 0.15 g/kg. The sequence of N and K application also could have influence on rice growth and nutrients uptake, especially when high rate of K applied, which could result in the reduce of rice growth and nutrients uptake caused by the decrease of available N in soil.