Abstract: Pistachio is a potential nut tree in the north and west of China, however, the micropropagation of pistachio is not still successful, this experiment were designed for establishing a tissue culture techniques of pistachio. Shoots collected from grafted tree (7yrs)of pistachio were used as explant in primary culture with different treatments of medium(MS, modified MS,Q-L,DKW), plant hormone(BA,IBA,NAA,GA), disinfectant(7%NaClO3, 0.1%mercuric chloride), addition(folic acid, calcium D-pantothenate and LH), glucose and sucrose, experiment results shown that explant of pistachio were disinfected well with 0.1% mercuric chloride for 10min, the suitable primary medium for pistachio was: 1/2DKW+6-0.5mg/l 6-BA +0.5mg/l IBA +3.0 mg/l GA3 +1mg/l folic acid+ 300mg/l LH +1mg/l calcium D-pantothenate+15g/l glucose+6.5g/l agar.