Establishment of System with High Frequency for Genetic Transformation of Populus tomentosa-85

Abstract

Abstract: Based on the established leaf explant regeneration system of Populus tomentosa-85, Several factors affecting transformation of P. tomentosa 85 were studied by infecting with Agrobacterium tumefaciens LBA4404, such as pre-culture time, the concentration of agroinfection, infection time, co-culture time and addition of acetosyringone(AS). Which the rate of kanamycin resistance plantlet was the measure index. An effective protocol in optimized conditions which were used MS dilute activated agroinfection for 10 times, 5minutes for infection and 4 d for co-culture medium for transformation of leaf disc of P. tomentosa 85 was developed and the frequency of transformation reached to 40.0%. The results laid a sound foundation for breeding new resistant species of P. tomentosa 85 gene transformation by means of leaf disc method.

CLC Number:

Q819.2

Li Keyou, Fan Junfeng, Zhao Zhong, Li Ling. Establishment of System with High Frequency for Genetic Transformation of Populus tomentosa-85[J]. Chinese Agricultural Science Bulletin, 2007, 23(7): 171-171.

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