Abstract: Vegetable is the indispensable and important foodstuff in our daily life because of its abundant vitamin, minerals, carbohydrate, protein, and so on. With the development of our society and rising of living standard, there is an increasing concern about the nutritive and safety quality of vegetables for researchers, producers and consumers. Lettuce with rich nutrients is broadly cultivated around the whole years in both north and south of China. In order to select best slow release fertilizer for high yield and good quality of lettuce, a pot experiment was conducted to study the effects of different slow release fertilizer (SR1, SR2, SR3, SR4) on the yield and quality of lettuce. There was significant difference between the yield of lettuce. The yield of lettuce is the lowest in SR3 treatment and is increased by 9.1%~12.7% compared with SR3 treatment, and the yield was in the order of SR4＞SR2＞SR1. The content of chla of lettuce was higher than that of chl b and the content of chla was in the order of SR3>SR2>SR4>SR1, but the content of Chlb and Chl (a+b) was SR3>SR2>SR1>SR4, and Chl (a/b) was SR4>SR3>SR2>SR1. The content of nitrate, soluble sugar and amino acids were in the order of SR1>SR2>SR3>SR4, the effect of different slow release fertilizer on the vitamin C of lettuce is not significant. Taking yield and quality into consideration, the optimum slow release fertilizer is SR2.