Abstract: The short Carnation (code number is SJ-3) is a fine variety witch was cultivated by hybridization of the Carnation and the Japanese dianthus. The variety has some characters of dwarf, double petals, no seed and cannot carry out sex-reproduction. Accordingly, the speed propagation experiment was conducted to study by tissue culture technology.The tissue culture of stem point section from short Carnation was studied. The results showed:(1)The suitable medium for induction culture and multiplication of the short Carnation was MS+6-BA 0.2mg/L+KT0.1mg/L+NAA0.05mg/L+CCC5ml/L;(2)The suitable rooting medium was improved MS+ NAA0.5 mg/L +IBA0.5 mg/L, with rooting rate 86.6%.(3)Under the same level condition, the effect to overcome the short Carnation ventilation was obvious when uses the seal membrane. (4)The root－developed plantlets transplanted in the substrate mixed with perlite sand and peat (volume ratio of 1:1:1),the survival rate reaches above 93%.The survival rate of transplant was the highest by rooting outside the bottle and abbreviated the propagation periods of test tube seedling.