Abstract: Rehmannia mosaic virus (ReMV) is probably a new species of Tobamovirus. The biological characteristics of ReMV were detected. The results revealed that the ReMV particle is rod-shaped and it could infect 10 plant species in 4 families. ReMV causes necrotic lesions on hosts such as Nicotiana glutinosa, Nicotiana rustica, Datura stramonium, Chenopodium quinoa, Chenopodium amaranticolor and Tetragonia expansa and causes systemic mosaic on hosts such as Nicotiana tabacum L, Nicotiana tobacum CV. K326, Lycoporsicon .esculentum and Brassica pekinensis. Its thermal inactivation point (TIP) ranges from 90℃ to 95℃, the dilution end point (DEP) is 10-5-10-6 and the longevity in vitro (LIV) was more than 60 days in 20-22℃.