Abstract: This item was instituted to screen out more tobacco germplasm resistant to main diseases and high leaf quality for breeding to use. 11 flue-cured tobacco germplasm resources with good comprehensive characters has been screened out from 125 germplasm resources in the field test in 2004, and then tested their main resistant by inoculated in the field or in greenhouse, and comprehensive evaluation with the control of K326 in the field designed by 3 times and randomized plot in the next year. The result shown that there were the germplasm resources of Huqiandajiwei, Zhubuo No.1 resistance to TMV, and the germplasm resources of Cg7900454, 6251, Jinyan No.6, Gejaji, and Jiyin No.1 media resisted to TMV, and 3 resistance to other primary disease. And also screen out 7 germplasm resources with low nicotine within 1% to 2%. Jiyin No.1, Zhubuo No.1, 85-5006 have good economic trait and good leaf quality. Huqiandajiwe, Woulihuang 0782 have budded 8 days earlier than K326,and Gejaji budded 5 days later than K326 to be identified. Those special germplasm resources, especially which with lower nicotine, will be recommend to the breeder to be use. And the other germplasm resources were produced more seed to conservation to be use in the future.