Abstract: We have researched the relationship between soil enzymatic activity and soil fertility of 24 representative rubber plantations in Danzhou, Hainan province. The results are as follows: Soil enzymatic activities are variant in different rubber plantation. Activities of catalase, invertase and acid phosphatase have close positive correlation with the organic matter, total nitrogen and available nitrogen, some of them are very remarkable; Activities of polyphnol oxidase and urease have negative correlation with organic matter, available nitrogen, available phosphorus and available potassium.