Abstract: The effects on the yields of flue-cured tobacco，activities of main soil enzymes were analyzed. By completely random design, the results with six seasons of continuous cropping showed that: (1)The yields of flue-cured tobacco in two soils were obviously reduced with increasing cropping year; Lime stones and clays formed in quaternary period also showed a significant deviation in the same cropping year. (2) The activities of urease, acid phosphatase, invertase became lower with continuous cropping, and the activity of catalase was increased significantly. (3)Continuous cropping problems of flue-cured tobacco in two soils were most obvious after four or five years; Lime stones was more suitable for continuous cropping of flue-cured tobacco than clays formed in quaternary period.