Abstract: 【OBJECTIVE】the soybean meal 、EFFSB and roasting soybean were used in an 【METHOD】in vitro rumen gas production method to imitate the dissimilarity under the milk cow TMR(DM) conditions. The influence of adding (0,5,10,15,20%) to ruminant in vitro gas production and the dry matter digestibility (DMD) and rumen fermentation parameters were determined. 【RESULTS】: The actual cumulative gas production in vitro assume the gradual trend of escalation with the time extension, but from 64 to 72 hours period, actual gas production in vitro remained basically unchanged.；under entire mix daily ration condition, after amounts of soybean meal reach 10%, it will alter the rumen fermentation characteristics, therefore increasing Rumen protein content, need to consider energy nitrogen balance；under the same raw material different gradient condition, adding EFFSB and roasting soybean to fermentation parameters and dry matter digestibility of rumen and the impact was not significant, explained the addition of energy and protein in TMR daily ration is beneficial on rumen healthy.Under the different raw material same gradient condition, all kinds of materials to rumen fermentation parameters of no significant impact. But B: C ratio in soybean meal and EFFSB, roasting soybean exist the opposite tendency, 【CONCLUSION】adding with 15% is the most notable.