Abstract: This experiment was done in the 30 years fixed position experiment of non-calcareous fluro-aquic soil in 2007. The effects of long-term located fertilization on dynamic changes of total S were studied. The results indicated that: the contents of soil total Sulphur was improved in the treatment which application of inorganic nitrogen fertilize, organic fertilizer and combined application of organic fertilizer and inorganic nitrogen fertilizer and combined application of of nitrogen and phosphorus and potassium fertilizer.But the more organic fertilizer and inorganic nitrogen fertilize was used, the more contents of soil total Sulphur was increased.The contents of soil total Sulphur in the treatment of the higher organic fertilize and the lower organic fertilize was improved average 39.9% and 20.2% respectively,compares with the CK.It can be increased average 92.2%,115.6%,70.6% and 115.1% respectively in the treatment of combined application of higher organic fertilizer and higher inorganic nitrogen fertilizer and lower inorganic nitrogen fertilizer ,lower organic fertilizer and higher inorganic nitrogen fertilizer and lower inorganic nitrogen fertilizer compares with the CK. The contents of soil total Sulphur was increased 57.8% and 116.8% in the treatment of N2PK, N2P, but the accumulation of the total Sulphur was highest in the treatment of N2P compares with other treatments, it has attained by 883.3 mg.kg-1 and 265.2 mg.kg-1 in the 0-20 cm and 20-40 cm soil layers.