Abstract: An experiment was conducted to study the anatomical structure of abscission zone of soybean petiole at different abscission stages, which made into normal paraffin slice. Results show that the abscission zone locates at the base of the petiole, containing 20~25 layer cells, about 2mm thick, distinguished from the adjacent cells. No markable morphological change of the abscission zone was observed during abscission with light microscope.