Abstract: To produce group-specific antibodies againstβ-agonists and to establish enzyme-immunoassay for the production of multi-residue detection kit. Clenbuterol, salbutamol and ractopamine were conjugated to KLH and BSA respectively. Rabbit were injected to prepare antiserums. ELISA was established with the group-specific antibody that selected by test the across-reaction of these antiserums. ELISA for salbutamol and clenbuterol was established with the anti-salbutamol antibody which shows 100% and 120% across-reaction with salbutamol and clenbuterol. This immunoassay provided an analytical method and laid a foundation for multiresidue ELISA kit.