Abstract: The factors of effecting on shoot regeneration from leaves in pear were studied. These factors included genotype, basic media, plant growth regulators, ways of leaf inoculation and culture condition of dark and light. Genotype is the most important factor of effecting on shoot regeneration from leaves. The highest rate of shoot regeneration were reached from D’Anjou and Hosui, both reached 100%，the lowest is Yellow Gold pear, only 27.3%. Plant growth regulators needed for different genotypes reaching the highest regeneration rate were different. TDZ is more effective than BA for inducing shoot regeneration from Duckpear, and BA is more effective than TDZ for inducing shoot regeneration from Pyrus communis L. and P. pyrifolia Nak. Shoot regeneration is improved by culturing 3 weeks under dark. The ways of leaf inoculation on media had less effect on shoot regeneration.