Abstract: Comparison test was conducted in Yuxi Yunnan with 6 early mature flue-cured tobacco varieties and K326 as the check, the objective of the test is screen tobacco varieties suitable for growing in Yunnan. By investigating the growing periods and the natural disease incidence in the field and analyzing the yield and the output, the results showed that among the 6 early mature varieties, 8902 and Islangold had the same growth period with the check, the nature of early mature did not showed up, while the other 4 varieties were 5~10d shorter in their growing periods, PVH19 showed pronounced early mature character and it was 10d earlier in growth period than the ck variety K326, Longjiang911 Xuandongqu and PVH19 had lower natural disease incidence in the field and better multiple disease resistance; Longjiang 911 and PVH19 had higher yields and output value than that of the ck; ratio of the grade A cured leaf of all the tested varieties were lower than that of the ck except PVH19 which was close to the ck. In summary of all the important characteristics of these varieties performed, the best was PVH19 followed by Longjiang91, the two varieties could be chosen for pilot growing in Yunnan tobacco area.