Abstract: The high gluten winter wheat xumai 27 was used as an experimental material to study the effects of spraying Fertilizers and bactericides on grain yield and quality. The results indicated that there were noticeable effects of spraying fertilizers and bactericides on grain 1000-kernel weight, yield and quality. Spraying macroelement or microelemen could increase 1000-kernel weight, yield and improved wheat quality. There were different effects of spraying different medicaments on grain 1000-kernel weight, yield and quality of xumai 27. Perhaps there were interaction in fertilizers and bactericides, so spraying fertilizers and bactericides together should be used more cautiously. The best effect could be gained by spraying macroelement, microelement and penicillin together in this treatment. 1000-kernel weight, yield and total evaluation were increased obviously.