Abstract: To find out the character of content of main chemical components on flue-cured tobacco，it were measured analyzing that 327 samples graded C3F from planting areas in Yunnan province. The studies were showed that Yunnan tobacco leaf characterized rich sugar，its total sugar and reducing sugar were 30.96% and 26.81% respectively. And CVs were much higher with chlorine，TS-RS，potassium-chlorine ratio，starch，sugar-nicotine ratio and citric acid,and it is more effected by ecological condition and planting tcehnic. And it were definitude which the distributing characteristic of frequency to thirty chemical components on Yunnan tobacco leaf. The disquisition results are important reference that the development of tobacco with characteristics and the resource collocating of tobacco leaf in Yunnan Province.