Abstract: Wild plants Premna microphylla Turcz. is of great development value. In order to understand the growth potential and inducing characteristics of the adventitious roots in winter, Premna was cultured via soil culture or solution culture in the room in winter. The results showed that Premna branches in the winter have rooting potential, and the induction of root was greatly decided by the environmental factors. Under the same temperature and light, Premna branches by soil culture sprouted without rooting. Compared to that, Premna by solution culture has not only grown sprouts, 10 percent of the plants induced adventitious root. In solution culture, balanced mineral nutrition can promote root growth and induction. For example, Premna cultured in 1/10 Hoagland nutrient solution was induced roots 6d earlier than that without any mineral elements, and the quantity was more than3.3. By solution culture Premna has the root of stout, very few branches. Premna rooting for auxin-induced effect was not obvious.