Abstract: In laboratory, the toxicity effects of six commercial fungicides on Botrytis cinerea were tested. The results of toxicity tested show that the EC50 of Prochloraz, Carbendazim, Procymidone, Iprodione, Diniconazoleon and Triadimefon on Bortrytis cinerea are 0.0233 mg/L、0.0421 mg/L、0.1724 mg/L、0.2837 mg/L、0.3021 mg/L and 0.3263 mg/L respectively. The EC50 of Prochloraz on Bortrytis cinerea is the highest among the tested fungicides, which shows that Bortrytis cinerea is the most sensitive to Prochloraz.