Abstract: replaced fluorescent lamp will be a major source for a wide range of plant tissue culture in future. The objective of this paper was to investigate the effect of light qualities on growth of chrysanthemum in vitro under light emitting diodes (LEDs), so as to provide dates support and theoretical basis on research of special light source in plant tissue culture.Combination the monochromatic light: red、blue、far-red and green generated by LEDs, Morphological characteristic, rooting, pigment content, carbon-nitrogen metabolism and antioxidative enzymes activities of chrysanthemum plantlets breeding 32-12 were compared with fluorescent lamp.The results showed that greatest stem length and the highest energy efficiency was obtained under monochromatic red light. Under blue light, the plantlets were low and strong, and root vigor was highest. Plants grown in complex of LEDs showed normal growth. Pigments under RBG were higher than other treatments. Red light promoted the contents of Chlb, blue light promoted the contents of Chla. The contents of starch in leaf is highest under monochromatic red light, RBG light stimulated the accumulation of soluble sugar、carbon hydrate、Amino acid Soluble . Blue light benefits to synthesize protein. Under LEDs, C/N ratios is higher than fluorescent lamp,With further improvements to be made, it is reasonable to expect that the total LEDs irradiation system