Abstract: Abstract: To study distribution and diversity of Bacillus thuringiensis in the different ecological area,806 soil samples were collected from Fuping Tianshengqiao, Laiyuan Baishi Moutaion ,Anxin Baiyang Lake, crop field in suburb of Baoding , herbs field in Anguo of Hebei. Forty six strians were isolated by temperature-antibiotic method. Eight kinds of cry gene, cry1, cry2，cry3，cry4，cry8，cry30 and cry32, were found by using PCR-RFLP, 11 Bt strains harbour unknown cry genes. According to SDS-PAGE analysis, results show that those Bt strains mainly express 130-150KD, 70-80kD, 60kD, 30kD protein.